Fig. 5: GFP-Pga59 is assembled within amyloid structures in adherent cells.
a Adhesion of C. albicans strains expressing either GFP-Pga59, GFP-Pga59V32,33N or the empty vector was triggered with magnetic beads and non-adherent cells were used as a negative control. Amyloid structures were enriched using a cell fractionation protocol developed to extract amyloids from yeast crude extracts. The resulting fibres were subsequently dropped on a copper grid, labelled with both the α-GFP antibody and gold particles prior to their negative staining with uranyl acetate. Micrographs are representative of the amyloid content in each condition. Scale bar: 200 nm. The right panels are a 2.5X enlargement of the adhesion columns from the left part (scale bar: 80 nm). Pga59 positive fibres are indicated with red arrows (right part). b The presence of amyloid structures after the cell fractionation procedure was assessed with ThT staining. Quantification of the ThT fluorescence in each condition is reported on the histogram (n = 3). The average values ± SD are used to present the data. (*) p < 0.05, (***) p < 0.001. The significance of the ThT fluorescence differences were tested using Student’s t-test. Source data are provided as a Source Data file.
