Fig. 6: Pga59V32,33N impairs adhesion on biotic and abiotic surfaces as well as biofilm establishment in C. albicans.
a Adhesion to abiotic surfaces was assessed by cultivating the indicated strains with magnetic beads. The formation of cellular aggregates was assessed by transmission microscopy (Nomarski). Scale bar: 200 μm. b The indicated strains were cultivated on a monolayer of Caco-2 cells to monitor adhesion on biotic surfaces. After 1 h of adhesion, C. albicans cells were washed and observed under the microscope (Nomarski). Scale bar: 10 μm. c C. albicans adhered on Caco-2 cells were counted on 200 µm2 areas. The experiment was repeated three times. Significance was assessed using Student’s t-test. (****) p < 0.0001. d Biofilms of the indicated overexpression strains were grown in a continuous flow microfermentor in a GHAUM medium in the presence or absence (negative control) of 50 μg mL−1 doxycycline. After 40 h, the biofilms were detached from the spatula, dried for 24 h, and weighed. The histogram presents the quantification of data derived from three independent experiments. Values are the average of each condition ± SD. (**) p < 0.01. The significance of the dry mass differences between the conditions was assessed using Student’s t-test. Source data are provided as a Source Data file.
