Fig. 7: Amyloid assembly and biofilm formation are impaired upon treatment with the Pga59 D1 V32,33N peptide. | npj Biofilms and Microbiomes

Fig. 7: Amyloid assembly and biofilm formation are impaired upon treatment with the Pga59 D1 V32,33N peptide.

From: The Pga59 cell wall protein is an amyloid forming protein involved in adhesion and biofilm establishment in the pathogenic yeast Candida albicans

Fig. 7: Amyloid assembly and biofilm formation are impaired upon treatment with the Pga59 D1 V32,33N peptide.

a Amyloid assembly upon adhesion was assessed by incubating a WT strain of C. albicans with magnetic beads, ThT and either the Pga59 WT D1 peptide (middle panels) or the V32,33N mutant version (bottom panels). Adherent cells without peptide treatment were used as a control (top panels). The ThT fluorescence was observed with an epifluorescence microscope. Scale bar: 25 μm. The experiment was performed three times. b Biofilms of the WT C. albicans strain were grown in TPP® six-well culture plates in RPMI medium in the presence of either the Pga59 WT peptide or the V32,33N peptide. Biofilms incubated without Pga59 peptides were used as a control. After 48 h, biofilms were washed, dried, and subsequently weighed. For each experimental condition, values are average ± SD. (**) p < 0.01. The quantification of three independent experiments is presented on the histogram. The significance of the results was evaluated using a Student’s t-test. Source data are provided as a Source Data file.

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