Fig. 2: Analysis of expression of the ebfG-operon by flow cytometry using reporter strains. | npj Biofilms and Microbiomes

Fig. 2: Analysis of expression of the ebfG-operon by flow cytometry using reporter strains.

From: Cell specialization in cyanobacterial biofilm development revealed by expression of a cell-surface and extracellular matrix protein

Fig. 2

a Number of cells as a function of fluorescence in cultures grown in fresh medium (FM) for 6 days. Strains analyzed: WT, PilB::Tn5 and their cognate reporter strains that bear a fusion of the regulatory region of the ebfG-operon with a yellow fluorescence protein (YFP). Arrows indicate fluorescence cutoff for calculating mutant cells with lower or higher expression of ebfG-operon compared to WT. b Number of cells as a function of fluorescence in PilB::Tn5/reporter cells grown in FM and conditioned medium (CM). c Fraction of PilB::Tn5/reporter cells with lower or higher expression of ebfG-operon compared to WT/reporter. Shown are averages and standard deviations from three independent experiments. d Biofilm (BF) formation by PilB::Tn5/reporter cells grown in FM or CM harvested at different time points of WT culture.

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