Fig. 5: Visualization of EbfG4::FLAG in permeabilized cells by immunocytochemistry and confocal fluorescence microscopy.

Strains analyzed: PilB::Tn5/EbfG4Ω/Comp and PilB::Tn5/EbfG4Ω/EbfG4::FLAG. Red represents autofluorescence whereas green indicates presence of EbfG4::FLAG. The scale bars correspond to 10 µm.