Fig. 6: Amyloid prediction and heterologous expression of EbfG1-4 proteins. | npj Biofilms and Microbiomes

Fig. 6: Amyloid prediction and heterologous expression of EbfG1-4 proteins.

From: Cell specialization in cyanobacterial biofilm development revealed by expression of a cell-surface and extracellular matrix protein

Fig. 6

a Prediction of amyloidogenic hotspots in the sequences of EbfG1-3 using six software prediction tools. Shaded fragments correspond to consensus positive prediction in at least four of the predictors. b Colony color phenotype of EbfG-expressing Escherichia coli compared to negative (Sup35[M]) and positive (Sup35[NM]) controls for amyloid formation in inducing plates. c Normalized red component of the brim of each colony as analyzed in ImageJ, based on five biological replicates and displayed as the mean and 95% confidence interval (t distribution).

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