Fig. 5: Treatment of preformed biofilms and bacterial viability of LL-37 and/or Ply2660. | npj Biofilms and Microbiomes

Fig. 5: Treatment of preformed biofilms and bacterial viability of LL-37 and/or Ply2660.

From: Bactericidal synergism between phage endolysin Ply2660 and cathelicidin LL-37 against vancomycin-resistant Enterococcus faecalis biofilms

Fig. 5

Bactericidal activity of LL-37 and/or Ply2660 against preformed biofilms of E. faecalis isolates a V583 and b Y15. Biofilms were allowed to develop for 24 h and then treated with LL-37 and/or Ply2660 for 6 h at 37 °C. Bacteria without any treatment served as a control. Results are expressed as the number of viable bacteria [in log10 CFU/well]. The data are presented as the means ± SD from two independent assays with three biological replicates in each assay; error bars represent standard deviation. Statistical significance was calculated using one-way ANOVA followed by Tukey’s multiple comparison test. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant. c Representative confocal microscope images of LIVE/DEAD-stained E. faecalis Y15 in 24-h-established biofilms after treatment with the indicated concentrations of LL-37, Ply2660, or a mixture of both at 37 °C for 6 h.

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