Fig. 6: Bacterial loads in the organs of E. faecalis-infected mice treated with LL-37 and/or Ply2660. | npj Biofilms and Microbiomes

Fig. 6: Bacterial loads in the organs of E. faecalis-infected mice treated with LL-37 and/or Ply2660.

From: Bactericidal synergism between phage endolysin Ply2660 and cathelicidin LL-37 against vancomycin-resistant Enterococcus faecalis biofilms

Fig. 6

a Survival curves of mice infected intraperitoneally with E. faecalis V583 (high dose, 1.8 × 109 CFU) and treated with a single dose of LL-37, Ply2660, a combination of LL-37 and Ply2660, or PBS via intraperitoneal injection (n = 12 for each group). For examination of bacterial loads in the b lung, c liver, and d kidney, mice were infected intraperitoneally with a lower dose (8 × 107 CFU) of E. faecalis V583 and treated as above (n = 5 for each group). The bacterial load was calculated by plating the samples on BHI agar. The data are presented as the means ± SD from five mice; error bars represent the standard deviation. Statistical significance was calculated using one-way ANOVA followed by Tukey’s multiple comparison test. **P < 0.01; ***P < 0.001; ns, not significant. e Bacterial detection (arrows) in tissue sections with H&E staining. Scale bars, 20 μm.

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