Fig. 4: Blastocystis ST1 colonization exerts a protective effect on DSS-induced colitis.

a Experimental design. b Scanning electron microscopy of colon and cecum tissues from ST1-colonized and un-colonized mice, ST1 are indicated with a red asterisk (*). Scale bar = 1 μm. c Blastocystis ST1 cells per milligram of stool in ST1-colonized mice. Weight changes (d) and DAI (e) between ST1-colonized and un-colonized mice during DSS treated for 7 days. f Representative micrographs of H&E-stained colon sections from un-colonized and ST1-colonized mice. Colon length (g) and colonic histological (h) in un-colonized and ST1-colonized group. Scale bar = 100 μm. Data are shown as the mean ± SEM. Significance was determined by two-sided unpaired Student’s t test (*P < 0.05; **P < 0.01).