Fig. 1: B. longum AO44 chloroform fractions induce splenocyte Interleukin-10 (IL-10) secretion. | npj Biofilms and Microbiomes

Fig. 1: B. longum AO44 chloroform fractions induce splenocyte Interleukin-10 (IL-10) secretion.

From: Extracellular vesicles of the Gram-positive gut symbiont Bifidobacterium longum induce immune-modulatory, anti-inflammatory effects

Fig. 1: B. longum AO44 chloroform fractions induce splenocyte Interleukin-10 (IL-10) secretion.

a Illustration of the bacterial growth and cells/supernatant separation. b Illustration of the bacterial conditioned media and cells chemical fractionation. c Fold change (compared to control) of IL-10 concentrations in splenocytes exposed to either of the chemical fractions generated from B. longum grown in BHIS media. d Fold change (compared to control) of IL-10 concentrations in splenocytes exposed to either of the chemical fractions generated from B. longum grown in GAM media. Each dot represents a technical repeat out of three independent experiments. Brown-Forsythe and Welch ANOVA, *P < 0.05 and **P < 0.01. Error bars represent s.d. CHCl3 fraction = hydrophobic organic fraction, MeOH fraction = hydrophilic organic fraction, H2O fraction = hydrophilic polar fraction, SupX10 = concentrated conditioned media. Figure 1a, b was created with BioRender.com.

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