Fig. 5: Immune-modulatory activity of B. longum AO44 extracellular vesicles. | npj Biofilms and Microbiomes

Fig. 5: Immune-modulatory activity of B. longum AO44 extracellular vesicles.

From: Extracellular vesicles of the Gram-positive gut symbiont Bifidobacterium longum induce immune-modulatory, anti-inflammatory effects

Fig. 5: Immune-modulatory activity of B. longum AO44 extracellular vesicles.

a IL-10 concentrations and b IL-17 concentrations in splenocytes media after exposure to anti-CD3; or anti-CD3 combined with concentrated BHIS (light gray, 1:50 dilution) or descending dilutions of extracellular vesicles. c CD4+ Ki67+ PD1+ cell frequency out of total CD4 cells after exposure to anti-CD3; anti-CD3 combined with concentrated BHIS (1:50 dilution) or descending dilutions of extracellular vesicles. d CD8+ Ki67+ PD1+ cell frequency out of total CD8 cells after exposure to anti-CD3 or anti-CD3 combined with concentrated BHIS (1:50 dilution) or descending dilutions of extracellular vesicles. e, f Fold change (compared to control) of IL-10 (e) or IL-17 (f) concentrations in DC-CD4+ T cells media exposed to either B. longum EVs or concentrated BHIS. Each dot represents a biological repeat out of three independent experiments. a–d Ordinary one-way ANOVA, e, f unpaired t test. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. Error bars represent s.d.

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