Fig. 4: Transcriptomics analysis of SOS, RpoS, and RpoH stress responses and of selected metabolic pathways. | npj Biofilms and Microbiomes

Fig. 4: Transcriptomics analysis of SOS, RpoS, and RpoH stress responses and of selected metabolic pathways.

From: A three-colour stress biosensor reveals multimodal response in single cells and spatiotemporal dynamics of biofilms

Fig. 4

RGB-S E. coli cells were treated with 2-propanol 2% (v/v) and sorted using FACS. Populations were sorted based on individual cell responses into four subpopulations, mono GFP, mono RFP, dual RFP-GFP, and triple RFP-GFP-BFP, with numbers (1, 2) indicating replicates. Differentially transcribed genes involved in SOS (a), RpoS (b), and RpoH (c) stress response pathways were analysed within the four sorted subpopulations. d Analysis of differentially transcribed metabolic pathways as annotated in the KEGG database of the four sorted subpopulations.

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