Fig. 6: Fermentation has a cardinal role in antimicrobial production.

A Colonies of LGG, S. typhimurium, E. faecalis, and P. aeruginosa, grown on solid TSB medium or TSB supplemented with glucose (1% W/V) or raffinose (1% W/V). The colonies were incubated at 37 °C, in a CO₂ enriched environment for 7 days. Colonies of LGG were inoculated next to S. typhimurium, E. faecalis, or P. aeruginosa colonies at a distance of 0.2 cm. B–D Colony-forming unit counts of E. faecalis or S. typhimurium or P. aeruginosa grown in 30% CM > 3 kDa or <3 kDa from LGG grown in TSB medium or TSB supplemented with glucose (1% W/V) or raffinose (1% W/V). Statistical analysis was performed using Brown-Forsythe and Welch’s ANOVA with Dunnett’s T3 multiple comparisons test. p < 0.05 was considered statistically significant. Growth curves of E E. faecalis and F S. typhimurium in 96 well plates in 37 °C with shaking. Cells were supplemented with 30% CM > 3 kDa from LGG grown in TSB medium supplemented with glucose (1% W/V) or 30% CM > 3 kDa from LGG grown in TSB medium supplemented with glucose (1% W/V) after proteinase K treatment (PK). Growth curves of G E. faecalis, H S. typhimurium in 96 well plates at 37 °C with shaking. Cells were supplemented with 30% CM < 3 kDa from LGG grown in TSB medium supplemented with glucose (1% W/V) or 30% CM < 3 kDa from LGG grown in TSB medium supplemented with glucose (1% W/V) after proteinase K treatment (PK). I Protein relative abundance between the sample of Msp1/p75 and J Msp2/p40. Statistical analysis was performed using Student’s t tests followed by FDR correction. p < 0.05 was considered statistically significant.