Fig. 5: Bacillaene inhibits curli production still when expression of curli subunit genes is uncoupled from its natural regulation. | npj Biofilms and Microbiomes

Fig. 5: Bacillaene inhibits curli production still when expression of curli subunit genes is uncoupled from its natural regulation.

From: Selective inhibition of the amyloid matrix of Escherichia coli biofilms by a bifunctional microbial metabolite

Fig. 5

a Macrocolonies of E. coli W3110 and derivatives ΔcsgD and ΔcsgBA mutants with or without expressing the csgBAC operon from a synthetic RpoS-dependent promoter (SynP(σs)), grown on CR-containing salt-free LB agar. The western blot reveals the cellular levels of CsgD in cells from respective macrocolonies. MW molecular weight. b, c Macrocolonies of W3110 and derivatives ΔcsgD and ΔcsgBA mutants with or without expressing the csgBAC operon from the SynP(σs) promoter, grown on CR-containing salt-free LB agar in close interaction with B. subtilis 3610 wt (b) or pksL mutant (c). The lower images show at higher magnification the zone of interaction between E. coli and B. subtilis strains. Scale bars in (a–c) represent 5 mm.

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