Fig. 6: Analysis of localization of CsgA in bacillaene-treated and untreated E. coli biofilms. | npj Biofilms and Microbiomes

Fig. 6: Analysis of localization of CsgA in bacillaene-treated and untreated E. coli biofilms.

From: Selective inhibition of the amyloid matrix of Escherichia coli biofilms by a bifunctional microbial metabolite

Fig. 6

a CsgA is found in low residual amount in E. coli W3110 macrocolonies experiencing inhibition of curli production. Biomass at the interaction and distant zones -as indicated in the images- was collected from E. coli macrocolonies grown in proximity to B. subtilis 3610 wt or pksL and subjected to SDS treatment and western blot analysis to detect CsgA. The SDS-PAGE gel is shown as a sample loading control. b CsgA is detected when bacillaene-treated macrocolonies devoid of curli fibers are sampled together with the underlying agar. Colony biofilms grown in the presence/absence of bacillaene or pksL extract were collected with the underlying pieces of agar and then subjected to SDS treatment and western blot analysis for CsgA detection. The SDS-PAGE gel is shown as a sample loading control. MW = molecular weight. Scale bars in (a, b) represent 5 mm.

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