Fig. 1: Inulin relieves HFD-induced MAFLD in laying hens.

A Representative photomicrographs of liver tissues with H&E staining (Scale bars = 200 µm) and phenotype pictures. B Body weight (kg). C Serum liver function index (U/L). ALT glutamic alanine transaminase, AST glutamic oxaloacetic transaminase. D Organ weight (g). E Organ index (%, organ weight (g)/body weight (g)*100%). F Serum lipid and glucose levels (mmol/L). TC total cholesterol, TG triglyceride, GLU glucose. G Hepatic lipid levels (mg/g liver). IHTG intrahepatic triglyceride, IHTC intrahepatic cholesterol. H LPS levels of liver tissue and cecum tissue (EU/g). I LPS levels of serum (EU/mL). J The relative mRNA expression of genes involved in the regulation of lipid transport and oxidation in liver tissues. K The relative mRNA expression of genes involved in the regulation of lipid synthesis in liver tissues. Data are presented as the mean ± SEM; n = 9–10 hens per group. Statistical analysis was performed using one-way ANOVA followed by the Least Significant Difference (LSD). *P < 0.05, **P < 0.01, ***P < 0.001.