Fig. 2: Daily fluctuation of the gut microbiota in the growing pigs.
From: Daily fluctuation of colonic microbiome in response to nutrient substrates in a pig model
a Dynamic change of the total bacterial load at each sampling time point within 48 h. # denotes a significant fluctuation of 24 h with a PAdj < 0.05. The rhythmicity analysis was finished by non-parametric JTK analysis with n = 8. All data were presented as mean ± s.d. b Venn diagram exhibiting the specific number of rhythmic genera identified in the relative abundance and the absolute abundance, respectively. c Heatmap depicting the relative abundance of each ASV with rhythmicity. The relative abundance of ASVs was normalized using Z-score methods. Row group annotation information from outside to inside represents its rhythmic fluctuation, corresponding phylum and family, respectively. ASV: amplicon single variant. Dynamic change of alpha diversity induce Richness (d) and Shannon (e) at different sampling time points. # denotes significant fluctuation with a PAdj < 0.05. The rhythmicity analysis was finished by non-parametric JTK analysis with n = 8. All data were presented as mean ± s.d. f Principal coordinate analysis (PCoA) plot of the bacterial community structure of samples from different time points based on the Bray-Curtis distance. The relative variable importance (R2) and significance (P) were calculated by PERMANOVA (Adonis) analysis with n = 8. P < 0.05 represents a significant difference between different sampling time points. g Correlations between colonic substrates and the Bray-Curtis distance of different sampling time points on PCoA1 and PCoA2 based on the Spearman correlation analysis method. * denotes a significant correlation with a P < 0.05. Composition and fluctuation of the key gut microbial taxa at the phylum (top 10, h) and genus (top20, i) level at different time points. # denotes significant rhythmic fluctuation with a PAdj < 0.05.
