Fig. 1: The microbial composition of the murine vaginal tract varies within colonies and between vivaria.
From: Vaginal microbial dynamics and pathogen colonization in a humanized microbiota mouse model
Vaginal swabs were collected from mouse colonies raised at BCM (n = 21), Jackson Lab (n = 71), and UCSD (n = 36, 12 mice repeatedly swabbed). a Vaginal microbial compositions of mice at BCM (blue bar), Jackson Lab (yellow bar), and UCSD (pink bar). b PCoA of vaginal communities clustered by weighted normalized UniFrac distances between mice at different vivaria. Centroids were determined by jackknifed rarefaction (100 reads). c Observed OTUs and (d) Shannon entropy of vaginal swab samples. e Bray-Curtis and (f) weighted normalized UniFrac distances between murine vaginal communities of mice within colonies (intra-site) at BCM, Jackson Lab, or UCSD. Colony instability and inter-site variation (weighted normalized UniFrac distances) in comparison to (g) BCM, (h) Jackson Labs, and (i) UCSD. j ANCOM results identifying unique taxa according to colony origin colored by BCM (blue), Jackson Lab (yellow), and UCSD (pink), or BCM and Jackson Lab (black and white). Each column (a) or symbol (b) represents a unique vaginal swab community. Tukey’s boxplots are displayed (c−i). Data were analyzed by Kruskal-Wallis with a Dunn’s multiple comparison test (c−f) or PERMANOVA followed by PERMDISP (g−i). PERMANOVA P values (P < 0.1) are colored in red if PERMDISP was also statistically significant (P < 0.05). All statistically significant P values are reported.
