Fig. 1: The expression of Csu pili and poly N-acetyl glucosamine in mountain-like biofilm patches formed by A. baumannii on glass surface.

A Left panel: Representative Western blot of three independent experiments to show CsuA/B expression in biofilms formed by A. baumannii 17978 in glass tubes at different intervals of time. 1–24 h, 2–48 h, 3–72 h, 4–24 h, 5–48 h, 6–72 h. The arrowhead indicates equal distribution of proteins through a nonspecific band used as a loading control. Right panel: Quantification of the blots using image J software. Bars graph indicate mean +/– standard deviation (SD). B A confocal laser microscopic image of Hoechst 33342-labelled biofilm formed by A. baumannii 17978 on the surface of an 18-well chamber glass slide after 72 h. In the left image, a typical patch of biofilm is marked by the square of dotted white lines. The two size bars correspond to 10 µm. The image on the right shows a 3-D stake image of the spotted patch. C A single and multiple staked 2-D view of biofilm patches labelled with Hoechst 33342 and CsuA/B specific antibodies. The CsuA/B positive matrix is shown in red. The size bars correspond to 10 µm. D A single and multiple staked 2-D view of biofilm patches labelled with Hoechst 33342, CsuA/B specific antibody and WGA. The WGA-bound fluorescent matrix representing poly B-acetyl glucosamine is shown in green. The size bars in the images correspond to 10 µm.