Fig. 3: Fluorescent imaging of biofilms. | npj Biofilms and Microbiomes

Fig. 3: Fluorescent imaging of biofilms.

From: Responses of drinking water bulk and biofilm microbiota to elevated water age in bench-scale simulated distribution systems

Fig. 3: Fluorescent imaging of biofilms.The alternative text for this image may have been generated using AI.

Fluorescent confocal laser scanning microscopy images of drinking water biofilms. (top row) Biofilms stained only with SYTO9 as a projection from above; (second row) Biofilms stained with all three dyes as a projection from above; and (third row) Biofilms stained with all three dyes from an angle. Representative images were chosen from each of seven biofilm sampling events. All images in the figure are of biofilms from the same reactor (reactor 5). MBH stands for maximum biofilm height. Scale bars represent 20 μm. Green: Nucleic acids stained with SYTO9. Orange: Proteins stained with Sypro Orange. Pink: Polysaccharides stained with Concanavalin A, Alexa Fluor 647 conjugate. Yellow: Overlap of green and orange.

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