Fig. 8: Heatmaps of MAGs.

Normalized coverages (log2) of a subset of 50 MAGs. The subset contains MAGs that were either (i) one of the top 10 most abundant MAGs in each sample, (ii) significantly differentially enriched in the reactor or tap reservoir bulk water across phases, or (iii) significantly differentially enriched in reactor bulk water versus biofilm. The top panel represents the phase of the samples. The left heatmap and legend display normalized coverage. The right heatmap and legend display enrichment and presence of key functional pathways. The column ‘enriched_p1_or_p2’ is the log2-fold-change between the phases of significantly enriched bins, where <0 is enriched in Phase I and >0 is enriched in Phase II. The column ‘enriched_bulk_or_bf’ is the log2-fold-change between the sample types of significantly enriched bins, where <0 is enriched in bulk water and >0 is enriched in biofilm. The following columns are presence/absence of KO# in each bin related to amoA/pmoA or complete KEGG modules related to degradation of amino and nucleic acids. Sample AR4_162 is not included due to suspected cross-contamination (see Section “Sequencing Negative Controls and Cross-Contamination”). A heatmap of all 90 MAGs is included as Fig. S. 23.