Fig. 3: Microscopy images of native B. subtilis biofilms following the addition of exogenously purified EpsA-O or MSgg media. | npj Biofilms and Microbiomes

Fig. 3: Microscopy images of native B. subtilis biofilms following the addition of exogenously purified EpsA-O or MSgg media.

From: Bacillus subtilis EpsA-O: A novel exopolysaccharide structure acting as an efficient adhesive in biofilms

Fig. 3

The addition of MSgg media or 0.2% exogenous EpsA-O occurred in three stages: at inoculation, and after 4 and 7 hours of incubation. Mature biofilms were cultivated on agar-filled 3D printed chamber slides and observed in situ. a A 3D-reconstructed CLSM image of mature biofilms. b CLSM slices through the biofilm structure of ∆eps and wt biofilms. All CLSM images were captured using a silicon oil objective, with scale bars indicating 10 μm.

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