Table 3 1H and 13C chemical shift assignments of the sample SL1 obtained upon solvolysis of EpsA-O recorded in D2O at 600 MHz and 70 °C
Residue | Nucleus | Chemical shifts (ppm) a | |||||
|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | ||
→3)-α-d-GlcpNAc-(1→ | 1H | 5.14 | 3.92 | 3.90 | 3.47 | 3.61 | 3.77-390 |
(GlcNAc) | 13C | 96.2 | 52.4 | 75.3 | 68.6 | 73.0 | 61.2 |
→6)-α-d-Galp-(1→ | 1H | 4.92 | 3.80 | 4.00 | 4.17 | 4.53 | 4.01 |
(αGal) | 13C | 98.8 | 69.2 | 69.2 | 68.5 | 68.3 | 65.2 |
→3,4)-β-d-GalpNAc-(1→ | 1H | 4.70 | 4.09 | 3.82 | 4.32 | 4.10 | 3.77-390 |
(GalNAc) | 13C | 101.4 | 51.7 | 76.3 | 73.5b (72.6)b | 76.1 | 61.2 |
→3)-β-d-QuipNAc4NAc-(1→ | 1H | 4.65 | 3.82 | 3.81 | 4.00 | 3.47 | 1.13 |
(Q) | 13C | 102.8 | 57.2 | 75.3 | 54.8 | 71.1 | 17.1 |
β-d-Galp(3,4-S-Pyr)-(1→ | 1H | 4.59 | 3.60 | 4.32 | 4.15 | 4.03 | 3.77-390 |
(tGalP) | 13C | 102.3 | 74.4 | 79.1 | 75.3 | 73.5 | 61.2 |
→6)-β-d-Galp(3,4-S-Pyr)-(1→ | 1H | 4.54 | 3.51 | 4.21 | 4.15 | 4.32 | 4.19 – 4.05 |
(GalP) | 13C | 102.8 | 73.8 | 79.1 | 75.3 | 72.6b (73.5)b | 69.2 |
