Fig. 3: Representative images of beads trapped in P. aeruginosa ΔmucA biofilm microcolonies before, during and after treatment with NAC.

a An overlay of the green channel displaying GFP-labelled bacteria and the red channel showing fluorescent beads (circled) before treatment. b, c The overlay of green, red and bright-field images during and after treatment with NAC. The green fluorescence was absent during and after treatment indicating bacterial cell death. The beads are circled for visualisation.