Fig. 5: Swelling of the P. aeruginosa ΔmucA matrix correlates with the stiffening of the matrix.

a The change in the size of the P. aeruginosa ΔmucA biofilm microcolonies with time. The black and blue curves correspond to the change in size of P. aeruginosa ΔmucA biofilms treated with 10 mg/mL NAC at pH 2.5 and with pH adjusted to 7, respectively. Panels I, II and III correspond to before, during and after treatment, respectively. b Comparison of the change in elastic modulus with time. The average elastic moduli of the biofilm microcolonies before (panel I), during (panel II), and after (panel III) treatment with 10 mg/mL NAC at pH 2.5 (black line) or with 10 mg/mL NAC, adjusted to pH 7 (blue line). The p-values are obtained by comparing the values with those before treatment using one-way ANOVA (N = 3).