Fig. 3: DEHP interference with astrocytes in the arcuate nucleus of the hypothalamus and potential mechanisms underlying the increase in GnRH release by DEHP. | npj Biofilms and Microbiomes

Fig. 3: DEHP interference with astrocytes in the arcuate nucleus of the hypothalamus and potential mechanisms underlying the increase in GnRH release by DEHP.

From: Di (2-ethylhexyl) Phthalate decrease pregnancy rate via disrupting the microbe-gut-hypothalamic-pituitary-ovarian axis in mice

Fig. 3

A–C Representative graphs and statistical plots illustrating the immunofluorescence of GFAP (n = 6, Student’s t test, **p < 0.01, ***p < 0.001) and NRG1 (n = 6, short-term exposure group: Mann–Whitney U test, **p < 0.01; long-term exposure group: Student’s t test, ***p < 0.001) in various DEHP treatment groups and control groups. Scale bars: 50 µm. D Bar chart displaying the changes in Prostaglandin E2 (PGE2) protein levels in the serum of different treatment groups (n = 6, Student’s t test, ***p < 0.001). E, F Representative immunoblot and quantification showing changes in GFAP/β-actin and NRG1/β-actin in different DEHP treatment groups compared to controls. (n = 3, Student’s t test, *p < 0.05). G–I Representative immunoblot and quantification showing changes in phosphor-ErbB2/ErbB2, phosphor-PI3K/PI3K and phosphor-AKT/AKT in different DEHP treatment groups compared to controls. (n = 3, Student’s t test, *p < 0.05; **p < 0.01; ***p < 0.001).

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