Fig. 4: The importance of root endophytic microbiota in the promotion of root apoplastic Fe reutilization.

a, b Manhattan plot displaying taxonomic information of root-enriched and -depleted OTUs from Fe-limited treatments compared to those from normal treatments ((log2 (fold change) > |1 | , FDR-adjusted p value < 0.05). Dashed line indicated the FDR-adjusted p value threshold of significance (p = 0.05). Venn analysis of c root-enriched and d root–depleted OTUs. e Cladogram showing phylogenetic relationships between 130 endo-bacterial OTUs. Loops from the inside out, indicated 1) phylum; 2) Relative abundance (RA) of OTUs in pH 6.2 treatment; 3) RA of OTUs in pH 7.5 treatment; 4) RA of OTUs in pH 8.0 treatment; and 5) Correlations between the RA of OTUs and rREUapoFe. f RA of Ammoniphilus OTU5 across different treatments. Different lowercase letters indicated significant differences among different treatments using Duncan’s multiple range tests at p < 0.05 (n = 6). Error bars indicate standard deviation of the mean. g Linear regression relationships between the RA of Ammoniphilus OTU5 and rREUapoFe.