Fig. 5: L-citrulline can alleviate DNA damage in Sertoli cell line. | npj Biofilms and Microbiomes

Fig. 5: L-citrulline can alleviate DNA damage in Sertoli cell line.

From: L-citrulline protects testicular Sertoli cell function by mitigating DNA damage via the gut-testis axis of sheep fed a high-concentrate diet

Fig. 5

A Study design diagram for treating the TM4 cell line with Sucrose and citrulline. B The representative images of ROS immunofluorescence of TM4 cell line in each group. Citr, treated with Citr; Sucrose, treated with Sucrose; Sucrose +H2O2, treated with Sucrose and Citr. Scale bar = 50 μm. C The histogram represents the cell proliferation capacity of each indicated group. D Western blot of γH2AX and GAPDH in each indicated group. E, F RT-qPCR analyzing the expression of Pcna and Gtf2h5 in each indicated group (n = 3). G Schematic model illustrating the in vivo isolation procedure for Sertoli cells. H Immunofluorescence staining of SOX9 in primary Sertoli cells isolated from mouse testes. Scale bar = 100 μm. I Quantification of cell proliferation capacity across experimental groups. J, K Representative immunoblot and densitometric analysis of γH2AX (normalized to β-ACTIN) in each group (n = 3). L, M RT-qPCR analyzing the expression of Pcna and Gtf2h5 in each indicated group (n = 3). N, O RT-qPCR analyzing the expression of Claudin11 and Occludin in each indicated group (n = 3). Statistical analyses were performed by one-way analysis of variance (ANOVA) with Tukey’s test for multiple comparisons.

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