Table 1 Sample sizes and Bd prevalence per species per site within three localities: Mountain Maryland (MD), the Smithsonian National Zoo & Conservation Biology Institute (NZCBI) campus in Front Royal, VA (Front Royal CBI) and George Washington Jefferson National Forest (GWJNF) in Virginia

From: Novel antimicrobial peptides and peptide-microbiome crosstalk in Appalachian salamander skin

Species

Locality

Site code

Bd prevalence

Sample size acetylcholine

Sample size massage

Sample size transcriptomics

E. bislineata

Front Royal CBI

POSH

38%

8

--

--

E. bislineata

GWJNF

HOQ

20%

3

2*

2*

E. bislineata

GWJNF

TIH

0

4

--

--

E. bislineata

Mountain MD

DMP

0

5

--

--

E. bislineata

Mountain MD

SRBR

0

3

--

--

P. cinereus

Front Royal CBI

POSH

0

8

--

2

P. cinereus

GWJNF

FLAK

20%

4

--

1

P. cinereus

GWJNF

TIMR

0

3

--

--

P. cinereus

Mountain MD

SRBE

0

4

--

2

P. cinereus

Mountain MD

DMP

0

6

6

--

N. viridescens

Front Royal CBI

LEEP

18%

9

--

2

N. viridescens

GWJNF

GAR

92%

5

7 (2*)

2*

N. viridescens

GWJNF

WOF

100%

4

--

--

N. viridescens

Mountain MD

DMP

80%

5

--

--

N. viridescens

Mountain MD

SRR

100%

5

--

2

   

TOTAL =

76

15

13

  1. Skin secretions were elicited by acetylcholine injection or via massage and then analyzed via proteomics. Skin samples were collected and peptide profiled using transcriptomics. Asterisk (*) indicates skin secretions were collected via massage and then skin samples were collected for transcriptomics; all other samples were independent.