Fig. 1: Schematic summary of approaches used in this study.
From: Emergent roles of infant gut microbes during the utilization of human milk oligosaccharides
Graphical abstract of the study. A biological replication of a synthetic consortium of seven infant gut microbes using three HMOs was established. Specific bacterial species were selectively removed, and the system was characterized by culture growth, metabolite production, and HMO utilization. Metatranscriptome analyses were obtained for each condition. Multivariate analyses and correlation networks were obtained. Finally, a mathematical model consisting of a system of ordinary differential equations was constructed to quantify the degradation rates of complex sugars and enable comparisons across different experiments. These comprehensive analyses led to the development of a model describing interactions among the consortium’s diverse bacterial members. Figures were retrieved from BioRender.
