Fig. 3: Both fluorescent probes specifically label cellulose in IsoF and PssUMAF1058 biofilms grown in flow-cell chambers. | npj Biofilms and Microbiomes

Fig. 3: Both fluorescent probes specifically label cellulose in IsoF and PssUMAF1058 biofilms grown in flow-cell chambers.

From: High-resolution visualization of biofilm matrix development in space and time using fluorescent stains for cellulose

Fig. 3

A Representative epifluorescence micrographs of 3-days old wildtype and mutant IsoF biofilms. Bright Field, Grayscale, and corresponding cellulose fluorescence in the presence of compound (1), Fire LUT. Bar = 50 μm. B, C Representative epifluorescence micrographs and total fluorescence quantifications (n = 40) of 3-days old biofilms of the PssUMAF0158 wildtype and its derived polysaccharide mutants grown in the presence of compound (1). D, E Representative epifluorescence micrographs and total fluorescence quantifications (n = 20) of 3-days old biofilms of the PssUMAF0158 wildtype and its derived polysaccharide mutants in the presence of compound (2). Bar = 20 μm. Different letters indicate statistically significant differences (p ≤ 0.05) according to one-way ANOVA followed by Tukey post-hoc test.

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