Fig. 5: Fluorescent labeling reveals cellulose as a private good in IsoF flow-cell biofilms. | npj Biofilms and Microbiomes

Fig. 5: Fluorescent labeling reveals cellulose as a private good in IsoF flow-cell biofilms.

From: High-resolution visualization of biofilm matrix development in space and time using fluorescent stains for cellulose

Fig. 5

A Top, mixed biofilms of IsoF wildtype strains marked either with GFP (blue) or mCherry (magenta); cellulose fluorescence (yellow). Cellulose was stained with Compound (1). Note that homogenous mixed aggregates rarely occur. Bottom, mixed biofilms of the IsoF wildtype (GFP, blue) and cellulose mutant (ΔbcsA mCherry, magenta). Note that ΔbcsA cells do not develop stable aggregates. CLSM acquisitions. Bars = 50 μm. B Temporal development of mixed biofilms shown in panel A. The lack of cellulose production by ΔbcsA cells appears to destabilize the entire biofilm matrix, impacting the wild-type population. Epifluorescence acquisitions. Bar = 50 μm. C Quantification of individual fluorophores in mixed Wt:Wt biofilms over time. D Quantification of individual fluorophores in mixed Wt: ΔbcsA biofilms over time. The washed-away biofilm shows a significantly reduced cellulose production compared to the biofilm formed by the wild-type strains only.

Back to article page