Abstract
The coexistence and horizontal transfer of antibiotic resistance genes (ARGs) and virulence factor genes (VFGs) carried by urban wildlife represent an emerging form of biological pollution, constituting a significant threat to public health. We employed meta-omic approaches to evaluate the effects of host traits (sex, age, etc.), environmental factors (including geographical location and time), and diet (including food composition and antibiotic residues) on the bacterial, ARG, and VFG profiles of Vespertilio sinensis, an urban-dwelling bat. Our results demonstrate that the feces of V. sinensis harbor diverse ARGs and VFGs, but their genomic evidence for horizontal mobility in bacterial communities is limited. Notably, environmental changes over time and across geographical locations are associated with the ARG and VFG profiles, potentially due to the influence of pollutants in specific habitats. Dietary factors are associated with their dynamics through the microbiome, with antibiotic residues exerting selective pressure on ARG profiles. No significant impacts of sex, age, body size, and reproductive status on the gut microbiota, resistome, or virulome were observed. This study provides valuable insights into the ecological drivers of the gut microbiome, resistome, and virulome in bats, thereby contributing to our understanding of the public health risks associated with urban wildlife.
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Data availability
Sequence data that support the findings of this study have been deposited in the NCBI Sequence Read Archive (SRA) database with the primary accession code PRJNA1298781.
Code availability
The underlying code used for ARG-VFG-MGE contigs identification in this study is available in Zenodo and can be accessed via this link [https://doi.org/10.5281/zenodo.17628441].
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This work was supported by the National Natural Science Foundation of China (grant nos. 32430066 and 32171525). The funder played no role in study design, data collection, analysis and interpretation of data, or the writing of this manuscript.
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Long Huang: Writing—original draft; writing—review and editing; visualization; formal analysis; conceptualization. Ying-Ting Pu: Investigation; writing—review and editing. Yan-Hui Zhao: Investigation; writing—review and editing; visualization. Xiao-Yu Sun: Investigation; visualization. Yue Zhu: Investigation; writing—review and editing. Ya-Ping Lu: Investigation; visualization. Hai-Xia Leng: Investigation; visualization. Jiang Feng: Conceptualization; funding acquisition. Long-Ru Jin: Conceptualization; writing—review and editing; supervision. Ke-Ping Sun: Conceptualization; writing—review and editing; project administration; supervision; funding acquisition. All authors reviewed the manuscript.
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Huang, L., Pu, YT., Zhao, YH. et al. Diet and environmental factors jointly drive the gut microbiome, resistome, and virulome of urban bats. npj Biofilms Microbiomes (2026). https://doi.org/10.1038/s41522-026-00930-y
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DOI: https://doi.org/10.1038/s41522-026-00930-y


