Fig. 1
CLS are found in MAT and macrophages constituting CLS are pro-inflammatory. a Photomicrographs of CD68 stained tumor slides showing macrophages in isolated ring-like formations surrounding dying or dead adipocytes (indicated by red arrows). Density of CLS in breast fat tissue away from tumor border was scored as number of CLS divided by area of fat tissue. a ×25 magnification, red arrow: CLS, black arrow: tumor; b ×150 magnification; c ×300 magnification of CLS detected in the MAT of an obese patient (BMI = 30 kg/m2) exhibiting hypertrophied adipocyte (mean adipocyte diameter 82.09 µm). b IL6 expression was evaluated in 5 samples exhibiting CLS obtained from overweight patients (BMI from 26.4 to 29.5 kg/m2) (Samples 1 to 5) (×200 magnification). The figures below (numbered 1’ to 5’) show two-fold-magnified views of selected areas indicated by insets. Similar experiments were performed in samples without CLS obtained from normal weight patients (BMI from 17.9 to 24.8 kg/m2) numbered (6 to 10), with two fold-magnified views of selected areas (6’ to 10’)
