Fig. 2
Tumorigenicity and metastatic potential of metM-Wntlung cells. a Survival curve (time until tumor reached 1.5 cm in any direction) of C57BL/6 mice transplanted with increasing concentrations of metM-Wntlung cells into the fourth mammary fat pad (1 × 103, 2.5 × 103, 5 × 103, 10 × 103 15 × 103, or 20 × 103 cells per mouse; n = 12/group). b Resultant tumor weights of cells transplanted in (a). c Representative micrographs of metM-Wntlung mammary tumors stained with H&E (top panel, scale bar 100 μm) and proliferative marker Ki67 (middle panel, 50 μm), and lung tissue stained with H&E (bottom panel 500 μm) from mice transplanted with the number of cells indicated. Bar graphs show the average number of proliferative cells per field (six fields chosen at random, n = 6 tumors) and incidence of metastatic lung tumors, mean ± SD. White arrow indicates intratumoral adipocyte, black arrows indicate apoptotic cells
