Fig. 3
Gene expression analysis of metastatic vs. nonmetastatic M-Wnt cells and comparison with human metastatic breast cancer. a Heat map showing the top 5000 most variable probes in M-Wnt, metM-Wntlung and metM-Wntliver cells. b Heat map showing significantly different gene expression in metM-Wnt cell metM-Wntlung) and metM-Wntliver cells (q < 0.001, FC > 3 or FC < 0.33) compared with nonmetastatic M-Wnt cells. c Venn diagram showing overlap between significantly different gene expression in metM-Wnt cells (p < 0.05, q < 0.001, FC > 3 or FC < 0.33) compared with nonmetastatic M-Wnt cells with comparisons of primary and metastatic breast cancer datasets in Oncomine (p < 0.001). d RNA expression of EMT-related genes in E-Wnt, M-Wnt and metM-Wntlung cells. Graphs show mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, Student’s t-test. e Representative immunoblots showing protein expression of E-cadherin and N-cadherin, Snail and β-Catenin in E-Wnt, M-Wnt and metM-Wntlung cells, with β-Actin as a loading control
