Fig. 4
Effects of obese phenotype on systemic metabolism and metM-Wntlung tumor growth and metastasis. a Effects of diet-induced obesity (DIO) diet, relative to control (CON) diet, on body fat (i) and lean mass (ii) (n = 5/group). b Effects of DIO diet on metabolic hormones. (i) Serum leptin to adiponectin ratio and serum concentration of resistin (ii) and insulin (iii) (n = 5/group). c Body weight (i) and non-fasting blood glucose level (ii) of mice fed a control (n = 15) or DIO diet (n = 13). d Survival curve of mice fed a control or DIO diet after injection with 2.5 × 103 metM-Wntlung cells in 200 μl into the tail vein. Mice were euthanized upon signs of any distress. p = 0.083, Gehan-Breslow–Wilcoxon test. e Extent of macrometastases and micrometastases in mice from survival study (Fig. 4D), as determined by gross pathology and histopathology, respectively. Bar graphs show the percentage of animals displaying macrometastases (control n = 3/15; DIO n = 5/13) and micrometastases (control n = 4/15; DIO n = 7/13). Representative micrographs of H&E (top panel) or Ki67 (bottom panel) stained serial sections (4 μm apart) of lung tissues from mice fed a control or DIO diet, showing metM-Wntlung macrometastases (left panels) and micrometastases (right panels). Bar graphs show the mean ± SD, *p < 0.05, ***p < 0.001, Student’s t-test
