Fig. 3

Flowchart identifying potentially treatable patients using real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) and quantitative immunofluorescence (QIF). Biopsy (sample 1) is initial specimen and excision (sample 2) is alternative specimen from same patient. RT-qPCR and QIF assays were performed on biopsy (sample 1), the initial specimen. IG4,2+: 2018 American Society of Clinical Oncology/College of American Pathologists in situ hybridization group 4 and IHC 2+