Fig. 6

Compounds NB-55, NB-68, NB-71, and NB-73 suppress breast tumor xenograft growth and the expression of FOXM1-regulated genes. Human breast cancer DT22 cells were injected into the mammary fat pad of intact 7-week-old NSG mice and mice bearing DT22 tumors were dosed in a daily and then every other day from day 7 on with 100 mg/kg of NB-55 or control vehicle by oral gavage; or b with NB-68, NB-71, or NB-73 by s.c. injection at 40 mg/kg daily for 4 days and then 20 mg/kg every third day until day 13 and then 10 mg/kg every third day, or with control vehicle. Tumor volumes in Veh and compound treated animals were monitored (2-way ANOVA, Dunnett’s post-test, **P < 0.01). In c, animals were treated with low doses of NB-73 (5 or 10 mg/kg s.c. daily and then every other day after day 21). Volumes of tumors in Veh and compound treated animals were monitored (2-way ANOVA, Bonferroni post-test, ****P < 0.0001, n = 8 per group, error bars are ± SD). d At the end of treatments, tumors from panel C were processed for gene expression analysis by q-PCR. (t-test, *p < 0.05; **p < 0.01; ***p < 0.001; ****P < 0.0001, n = 8 per group, error bars are ± SD).