Fig. 2: Repertoire of genetic alterations in primary and metastatic mixed mucinous breast cancers.

a Representative photomicrographs of a H&E-stained primary mixed mucinous breast cancer (pMUC; top), and a metastatic mixed mucinous breast cancer (mMUC) involving liver (bottom). Scale bars in a, 100 μm (top) and 50 μm (bottom). b Boxplots depicting the non-synonymous tumor mutation burden in mMUCs (n = 5), metastatic invasive ductal carcinoma of no special type matched by age, menopausal status, and estrogen receptor (ER)/HER2 status (mIDC-NST; n = 15), and pMUCs (n = 14). Mann–Whitney U test, two-tailed. c Comparison of the cancer genes most frequently affected by non-synonymous somatic mutations, amplifications, or homozygous deletions in mMUCs (n = 5), in age-, menopausal status-, and ER/HER2 receptor status-matched mIDC-NSTs (n = 15), and in pMUCs (n = 14). Cases are shown in columns and genes in rows. Mutation types are color-coded according to the legend. ER/HER2 status are shown on phenobars (top). *P < 0.05; Fisher’s exact test, two-tailed. Indel insertion/deletion, LOH loss of heterozygosity, SNV single nucleotide variant.