Fig. 3: Quantitative immunofluorescence (QIF) analysis of murine B7-H3 expression in normal mammary tissue and glands containing DCIS.
From: Evaluation of ductal carcinoma in situ grade via triple-modal molecular imaging of B7-H3 expression
a Representative immunofluorescence (20×, top) and H&E (10×, bottom) stained normal murine mammary tissues (left) and those containing DCIS (right). Scale bars = 200 μm. b Box plot showing quantitative IF composite scores (a.u.) of normal breast tissue (n = 5, CS = 0.01 ± 0.02 a.u.) and DCIS (n = 7, CS = 45.96 ± 12.76 a.u.). Box plot follows Tukey rules. DCIS tissues have statistically significantly higher B7-H3 staining than normal murine tissue both on the endothelial and epithelial cells (P < 0.001). c Receiver operating curve depicting the ability of murine B7-H3 expression visualized by QIF to differentiate between normal and DCIS containing tissues (AUC = 1.00, 95% CI 1.00, 1.00).
