Fig. 4: Schema of QuPath image analysis steps.
From: Systematic evaluation of scoring methods for Ki67 as a surrogate for 21-gene recurrence score
a After entire invasive tumor area is identified as a region of interest using a hand drawing tool, 300 × 300 μm grids were generated to fill the region of interest. b After positive cell detection within each grid, cell classifier is generated based on annotation of representative tumor and host cells, and applied to all grids. c Magnified view of a single grid after cell classifier is applied (right); negative tumor cell nuclei (blue), positive tumor nuclei (red), stromal cell nuclei (green). d An example of within-tumor heterogeneity of ki67 staining with left side showing nearly 100% cells staining while right side show lesser staining.
