Fig. 2: Assessment of concurrent combination treatment regimens. | npj Breast Cancer

Fig. 2: Assessment of concurrent combination treatment regimens.

From: Treatment scheduling effects on the evolution of drug resistance in heterogeneous cancer cell populations

Fig. 2

a Cell growth curve for two different combination regimens. The schedules included two cycles, each consisting of a 3-day treatment period followed by a 10-day recovery period. Points on time axis: a-pre-treatment baseline, b-after 2 days treatment, c-after 3 days treatment, d-after 10 days of recovery, e-after 2 days of treatment in cycle 2, f-after 3 days of treatment in cycle 2, g-after 10 days of recovery in cycle 2. b Percentage of apoptotic cells using Annexin V and PI staining at different treatment points in the schedule. c Mammosphere count after the end of the treatment schedule. d Colony formation assay after the end of the treatment schedule. Representative pictures of colony formation are provided in Supplementary Fig. 2b. e Quantification by Western blot of protein levels involved in targeted pathways. In a–d, the error bars shown represent the standard deviation of triplicate measurements (biological replicates). P-values are from a two-sided t-test.

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