Fig. 3: Sequential regimens result in different patterns of resistance.

a Growth curves associated with sequential treatment regimens and schedules. Cells received a 3-day treatment followed by 2 days (top), 5 days (middle), or 10 days (lower) of recovery in cycle 1, followed by 3-day treatment and 10-day recovery in cycle 2. Left Panel: navitoclax given as cycle 1 treatment. Right panel: crizotinib given as cycle 1 treatment. b Quantification by Western blot of protein levels involved in targeted pathways at different times in the treatment cycle. Letters correspond to the time points on the plots in a. The colors identifying the different samples at each time point corresponding to the color of the curves in a.