Fig. 4: Effect of treatment schedules on cell response.

a Effect of cycle 1 treatment and duration on MDA-MB-231 cell growth. b Effect of the duration of drug-free recovery after cycle 1. Each group corresponds to a different duration of drug-free recovery and includes all cycle 1 schedules for the corresponding treatment regimen. c Effect of cycle 2 treatment and duration over all the combinations of cycle 1 treatment durations and drug-free recovery periods. d Effect of the duration of recovery after cycle 2, over all the other combinations of prior durations. Combination 1 included equal doses of navitoclax and crizotinib. Combination 2 included a higher dose of crizotinib than navitoclax. Throughout the plots, points have been color-coded based on the duration of cycle 1 treatment (vehicle—black, 1 day—red, 2 days—blue, 3 days—green). Since scales on panels a and b differ, we added a red reference line at 3000 to make the comparison easier. The box plots represent the distribution in the luminescence value, with the lower and upper sides of the box representing the first (Q1) and third (Q3) quartiles, the thick line representing the median, and the length of the upper and lower whiskers being 1.5(Q3-Q1).