Fig. 5: Density and spatial dispersion of isolated lymphocytes and isolated CD3⁺ T cells within stroma are significantly higher for good outcome tumors, but not for isolated stromal CD20⁺ B cells.

Density of isolated (a) lymphocytes, (b) CD20⁺ B cells, and (c) CD3⁺ T cells within stroma for good vs. poor outcome tumors. Plots of (d) occupancy AUC and (e) FD difference of isolated CD3⁺ T cells within stroma showing greater spatial dispersion in good compared to poor outcome. Plots of (f) occupancy AUC and (g) FD difference showing that spatial dispersion of isolated B cells within stroma is not significantly different between good vs. poor outcome. Patient cohorts for plots in this figure are n = 24 for good outcome (blue) and n = 12 for poor outcome (red). For all box-and-whisker plots, the center line represents the median, box limits represent the upper and lower quartiles, whiskers indicate maximum and minimum values within 1.5 interquartile ranges of the upper and lower quartiles; individual points are outliers. P-values in box plots are determined by unpaired, two-tailed Student t-tests. ns: not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 1 × 10⁻⁴.