Fig. 6: Downregulation of ECM factors in CEBPB-ko cells.
From: C/EBPβ isoform-specific regulation of migration and invasion in triple-negative breast cancer cells
a Expression of ECM-associated genes relative to GAPDH in wt BT-20 cells and two different clones of CEBPB-ko BT-20 cells (n = 3). Results are analysed using one-way ANOVA analysis. Error bars represent SD, **p < 0.01, ****p < 0.0001. b C/EBPβ-associated fragments associated with H3K acetylation (H3K27Ac). Data from the ENCODE database (http://genome.ucsc.edu/ENCODE/)(see Supplementary Table 1). c Immunoblots showing expression of Thrombospondin 1 (THBS1), Tenascin C (TNC), Fibronectin 1 (FN1) or Collagen 5A1 (COL5A1) in wt BT-20 cells and two different clones of Cebpb-ko BT-20 cells. Loss of C/EBPβ-LAP expression shows the successful CEBPB-knockout and β-actin was used for loading control.
