Fig. 2: ST8Sia2 and ST8Sia4 expression in primary breast tumors. | npj Breast Cancer

Fig. 2: ST8Sia2 and ST8Sia4 expression in primary breast tumors.

From: The different prognostic significance of polysialic acid and CD56 expression in tumor cells and lymphocytes identified in breast cancer

Fig. 2

A ST8Sia4 and ST8Sia2 in situ hybridization (ISH) and identification of ST8Sia2 (ii), ST8Sia4 (iii), and nuclei (iv) using Intellesis software. Red arrows point to (i) ST8Sia4 RNA punctae from ISH staining and (iii) ST8Sia4 RNA punctae as identified by Intellesis software. Green arrows point to (i) ST8Sia2 RNA punctae from ISH staining and (ii) ST8Sia2 RNA punctae as identified by Intellesis software. Scale bar = 10 µm. B and C Quantification of the total number of ST8Sia2 and ST8Sia4 RNA punctae as a ratio of total number of tumor cell nuclei (B) or infiltrating stromal cell nuclei (C). ±SEM, unpaired t test with Welch’s correction. D Representative image of a ST8Sia4 high expressing tumor. Black arrows point to ST8Sia4 RNA punctae in tumor cells and red arrows point to ST8Sia4 RNA punctae in infiltrating stromal cells. Scale bar = 20 µm. E and F ST8Sia4 expression in (E) tumor cells (luminal A, n = 39; Luminal B, n = 9; HER2+, n = 8; TNBC, n = 20) and F infiltrating stromal cells (Luminal A, n = 37; Luminal B, n = 6; HER2+, n = 8; TNBC, n = 20) grouped by molecular subtype. G and H ST8Sia4 expression in G tumor cells (Grade 1, n = 17; Grade 2, n = 51, Grade 3, n = 18) and H infiltrating stromal cells (Grade 1, n = 16; Grade 2, n = 48, Grade 3, n = 13) grouped by overall tumor grade. ±SEM, Kruskal–Wallis test.

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