Fig. 2: Different spatial distributions of various immunophenotypes were associated with diverse prognoses in Miller-Payne 4/5 patients.

a Immunophenotypes from all regions were standardly clustered by protein expression markers (total 33). Scaled from −2 to 2. b Heatmaps denote spatial proximity z scores between pairs of clusters (y-axis) in regions of all neighbors, scaled from −5 to 5. c t-SNE plots showed the similarity of immunophenotypes between compared groups (left), and divided immunophenotypes population according to clusters (right). d The different expression values of 33 resulting clusters (y-axis) between compared groups (x-axis) are shown, scaled from −1 to 1. e The random forest classifier assessed by receiver operating characteristic (ROC) curve with the area under the curve (AUC). f Heatmaps character the spatial distinction of immunophenotypes between Miller-Payne 4 groups with different prognoses. t-SNE t-distributed stochastic neighbor embedding. G4 Miller-Payne 4, G5 Miller-Payne 5.