Fig. 1: Effect of p53 missense mutations on hallmark phenotypes of cancer.
Phenotypes were measured in three independent experiments with replicates, and the results were normalized to the log2 fold change over the phenotype of p53 WTOE cells. The error bars represent the standard error of mean (SEM) values, and significant differences (two-sided single-sample t-test) from the WTOE mean are indicated by red asterisks for indicated p values. In all plots, cells were sorted by each phenotype, from less (left) to more (right) aggressive. a Survivability of the cell line panel in absence of growth factors (serum and EGF) were measured by the CellTiter-glo assay. b Cell apoptosis was detected by the level of activity of caspase 3 and caspase 7, and the reciprocal of luminescence were used to measure the level of resistance to apoptosis. c Migration was assessed using the transwell assay. d Cell invasion was assessed using the transwell assay with Matrigel-coated chamber membranes. e To measure anoikis, the death index (ratio of dead cells and live cells) was calculated for each cell line, and the reciprocal values were used to measure the level of resistance to anoikis.
